Factors affecting growth and extracelluar lipase production by Pseudomonas fluorescens 2D
Identifieur interne : 001159 ( Main/Exploration ); précédent : 001158; suivant : 001160Factors affecting growth and extracelluar lipase production by Pseudomonas fluorescens 2D
Auteurs : A. Makhzoum [Royaume-Uni] ; J. S. Knapp [Royaume-Uni] ; R. K. Owusu [Royaume-Uni]Source :
- Food Microbiology [ 0740-0020 ] ; 1995.
Abstract
Some of the factors affecting the growth and the production of extracelluar lipase by Pseudomonas fluorescens strain 2D were studied. Lipase production appeared to parallel the growth of the bacterium and lipase production had ceased by the onset of stationary phase. The organism grew optimally in the temperature range 15–30°C, while lipase production was optimum around 20°C. Growth and lipase production were better in peptone water than in other complex media. Among nitrogen sources, amino acids such as arginine, threonine and lysine supported good lipase production, as did some inorganic nitrogen sources such as ammonium salts of mineral acids. In the case of carbon sources, most amino acids tested did not serve as good carbon sources, resulting in poor growth. However, organic acids such as pyruvate and succinate supported good growth and lipase production. Most of the sugars repressed lipase production. For example, glucose supported good growth but completely repressed lipase production. Among lipid substrates, tricaproin and caproic acid enhanced considerably lipase production, while triolein and oleic acid repressed it strongly. High phosphate concentrations inhibited lipase production, while heavy metal ions such as mercury, copper, caesium, zinc, manganese and cobalt were very inhibitory. Calcium, on the other hand, had no effect on the growth and strongly stimulated (360%) lipase production. Addition of inhibitors of protein synthesis, chloramphenicol and rifamycin, caused immediate cessation of growth and lipase production.
Url:
DOI: 10.1016/S0740-0020(95)80108-1
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream Istex, to step Corpus: 000E98
- to stream Istex, to step Curation: 000A44
- to stream Istex, to step Checkpoint: 000993
- to stream Main, to step Merge: 001208
- to stream Main, to step Curation: 001159
Le document en format XML
<record><TEI wicri:istexFullTextTei="biblStruct"><teiHeader><fileDesc><titleStmt><title xml:lang="en">Factors affecting growth and extracelluar lipase production by Pseudomonas fluorescens 2D</title><author><name sortKey="Makhzoum, A" sort="Makhzoum, A" uniqKey="Makhzoum A" first="A." last="Makhzoum">A. Makhzoum</name></author><author><name sortKey="Knapp, J S" sort="Knapp, J S" uniqKey="Knapp J" first="J. S." last="Knapp">J. S. Knapp</name></author><author><name sortKey="Owusu, R K" sort="Owusu, R K" uniqKey="Owusu R" first="R. K." last="Owusu">R. K. Owusu</name></author></titleStmt><publicationStmt><idno type="wicri:source">ISTEX</idno><idno type="RBID">ISTEX:154E694EE9A38D8C3EEC6572CCE1B69B9DC49498</idno><date when="1995" year="1995">1995</date><idno type="doi">10.1016/S0740-0020(95)80108-1</idno><idno type="url">https://api.istex.fr/document/154E694EE9A38D8C3EEC6572CCE1B69B9DC49498/fulltext/pdf</idno><idno type="wicri:Area/Istex/Corpus">000E98</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Corpus" wicri:corpus="ISTEX">000E98</idno><idno type="wicri:Area/Istex/Curation">000A44</idno><idno type="wicri:Area/Istex/Checkpoint">000993</idno><idno type="wicri:explorRef" wicri:stream="Istex" wicri:step="Checkpoint">000993</idno><idno type="wicri:doubleKey">0740-0020:1995:Makhzoum A:factors:affecting:growth</idno><idno type="wicri:Area/Main/Merge">001208</idno><idno type="wicri:Area/Main/Curation">001159</idno><idno type="wicri:Area/Main/Exploration">001159</idno></publicationStmt><sourceDesc><biblStruct><analytic><title level="a" type="main" xml:lang="en">Factors affecting growth and extracelluar lipase production by Pseudomonas fluorescens 2D</title><author><name sortKey="Makhzoum, A" sort="Makhzoum, A" uniqKey="Makhzoum A" first="A." last="Makhzoum">A. Makhzoum</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Procter Department of Food Science, The University of Leeds, Leeds LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Knapp, J S" sort="Knapp, J S" uniqKey="Knapp J" first="J. S." last="Knapp">J. S. Knapp</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Department of Microbiology, The University of Leeds, Leeds LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author><author><name sortKey="Owusu, R K" sort="Owusu, R K" uniqKey="Owusu R" first="R. K." last="Owusu">R. K. Owusu</name><affiliation wicri:level="4"><country xml:lang="fr">Royaume-Uni</country><wicri:regionArea>Procter Department of Food Science, The University of Leeds, Leeds LS2 9JT</wicri:regionArea><orgName type="university">Université de Leeds</orgName><placeName><settlement type="city">Leeds</settlement><region type="country">Angleterre</region><region type="région" nuts="1">Yorkshire-et-Humber</region></placeName></affiliation></author></analytic><monogr></monogr><series><title level="j">Food Microbiology</title><title level="j" type="abbrev">YFMIC</title><idno type="ISSN">0740-0020</idno><imprint><publisher>ELSEVIER</publisher><date type="published" when="1995">1995</date><biblScope unit="volume">12</biblScope><biblScope unit="supplement">C</biblScope><biblScope unit="page" from="277">277</biblScope><biblScope unit="page" to="290">290</biblScope></imprint><idno type="ISSN">0740-0020</idno></series><idno type="istex">154E694EE9A38D8C3EEC6572CCE1B69B9DC49498</idno><idno type="DOI">10.1016/S0740-0020(95)80108-1</idno><idno type="PII">S0740-0020(95)80108-1</idno><idno type="ArticleID">95801081</idno></biblStruct></sourceDesc><seriesStmt><idno type="ISSN">0740-0020</idno></seriesStmt></fileDesc><profileDesc><textClass></textClass><langUsage><language ident="en">en</language></langUsage></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some of the factors affecting the growth and the production of extracelluar lipase by Pseudomonas fluorescens strain 2D were studied. Lipase production appeared to parallel the growth of the bacterium and lipase production had ceased by the onset of stationary phase. The organism grew optimally in the temperature range 15–30°C, while lipase production was optimum around 20°C. Growth and lipase production were better in peptone water than in other complex media. Among nitrogen sources, amino acids such as arginine, threonine and lysine supported good lipase production, as did some inorganic nitrogen sources such as ammonium salts of mineral acids. In the case of carbon sources, most amino acids tested did not serve as good carbon sources, resulting in poor growth. However, organic acids such as pyruvate and succinate supported good growth and lipase production. Most of the sugars repressed lipase production. For example, glucose supported good growth but completely repressed lipase production. Among lipid substrates, tricaproin and caproic acid enhanced considerably lipase production, while triolein and oleic acid repressed it strongly. High phosphate concentrations inhibited lipase production, while heavy metal ions such as mercury, copper, caesium, zinc, manganese and cobalt were very inhibitory. Calcium, on the other hand, had no effect on the growth and strongly stimulated (360%) lipase production. Addition of inhibitors of protein synthesis, chloramphenicol and rifamycin, caused immediate cessation of growth and lipase production.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country><region><li>Angleterre</li><li>Yorkshire-et-Humber</li></region><settlement><li>Leeds</li></settlement><orgName><li>Université de Leeds</li></orgName></list><tree><country name="Royaume-Uni"><region name="Angleterre"><name sortKey="Makhzoum, A" sort="Makhzoum, A" uniqKey="Makhzoum A" first="A." last="Makhzoum">A. Makhzoum</name></region><name sortKey="Knapp, J S" sort="Knapp, J S" uniqKey="Knapp J" first="J. S." last="Knapp">J. S. Knapp</name><name sortKey="Owusu, R K" sort="Owusu, R K" uniqKey="Owusu R" first="R. K." last="Owusu">R. K. Owusu</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Terre/explor/CobaltMaghrebV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001159 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 001159 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Terre
|area= CobaltMaghrebV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= ISTEX:154E694EE9A38D8C3EEC6572CCE1B69B9DC49498
|texte= Factors affecting growth and extracelluar lipase production by Pseudomonas fluorescens 2D
}}
| This area was generated with Dilib version V0.6.32. |  |